{"id":42679,"date":"2021-12-15T17:33:19","date_gmt":"2021-12-15T16:33:19","guid":{"rendered":"https:\/\/www.filtrox.com\/blog\/2021\/12\/15\/application-note-aufreinigung-einer-mak-produzierenden-zellkultur\/"},"modified":"2024-12-06T14:04:00","modified_gmt":"2024-12-06T13:04:00","slug":"application-note-aufreinigung-einer-zellkultur","status":"publish","type":"post","link":"https:\/\/www.filtrox.com\/ch\/blog\/2021\/12\/15\/application-note-aufreinigung-einer-zellkultur\/","title":{"rendered":"Application Note: Aufreinigung einer mAk-produzierenden Zellkultur"},"content":{"rendered":"\n

In der vorliegenden Application Note wird die Optimierung des Filtrationsprozesses einer mAk-enthaltenen CHO-Zellkultur im Detail gezeigt. Bislang wird die Suspension in drei Schritten filtriert, bevor eine Sterilfiltration mit einer 0.2 \u03bcm Membran durchgef\u00fchrt werden kann. Um die Sterilfiltration zu gew\u00e4hrleisten, muss vor jedem Filtrationsschritt ein Flockungsmittel zugegeben werden. Laborfiltrationstests mit FILTROX Tiefenfilterschichten werden durchgef\u00fchrt, um die Anzahl Prozessschritte bei der Zellernte zu reduzieren.<\/p>\n

Ziel ist es dabei, das am besten geeignete Filterhilfsmittel sowie deren ideale Menge zu bestimmen und Tr\u00fcbungswerte unter 30 NTU zu erreichen, um eine anschliessende Sterilfiltration zu garantieren.<\/p>\n

DIE APLICATION NOTE LESEN<\/strong><\/a><\/p>\n

\"zellkultur<\/p>\n","protected":false},"excerpt":{"rendered":"

In der vorliegenden Application Note wird die Optimierung des Filtrationsprozesses einer mAk-enthaltenen CHO-Zellkultur im Detail gezeigt. Bislang wird die Suspension in drei Schritten filtriert, bevor eine Sterilfiltration mit einer 0.2 \u03bcm Membran durchgef\u00fchrt werden kann. Um die Sterilfiltration zu gew\u00e4hrleisten, muss vor jedem Filtrationsschritt ein Flockungsmittel zugegeben werden. Laborfiltrationstests mit FILTROX Tiefenfilterschichten werden durchgef\u00fchrt, um … Continue reading Application Note: Aufreinigung einer mAk-produzierenden Zellkultur<\/span> →<\/span><\/a><\/p>\n","protected":false},"author":8,"featured_media":0,"comment_status":"open","ping_status":"open","sticky":false,"template":"","format":"standard","meta":{"_acf_changed":false,"advgb_blocks_editor_width":"","advgb_blocks_columns_visual_guide":"","footnotes":""},"categories":[456,104,509,470],"tags":[],"class_list":["post-42679","post","type-post","status-publish","format-standard","hentry","category-neuigkeiten","category-news-ch","category-news-life-science-ch-2","category-news-life-science-ch"],"acf":[],"yoast_head":"\nApplication Note: Aufreinigung einer mAk-produzierenden Zellkultur<\/title>\n<meta name=\"description\" content=\"In der vorliegenden Application Note wird die Optimierung des Filtrationsprozesses einer mAk-enthaltenen CHO-Zellkultur im Detail gezeigt.\" \/>\n<meta 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