Tag Archives: plasmid dna purification

PLASMID-DNA

Plasmid-DNA clarification, purification header

 

Optimized solutions for Plasmid- DNA clarification and purification

PURADISC® BIO SD is the state-of-the-art technology for alluvial filtration for Plasmid-DNA clarification. It is based on depth filters that capture contaminants in their three-dimensional matrix. The used PURAFIX® depth filters are low in extractable ions and pyrogens. By adding filter aid, such as diatomaceous earth, the capacity per m2 filter area can be drastically increased. 

process of purification and clarification of plasmid DNA

 

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The main advantage of alluvial filtration for the clarification step of Plasmid-DNA is the elimination of the two-step approach. Due to the increased filtration capacity the traditional approaches such as centrifugation followed by depth filtration or a two-step depth filtration are not necessary anymore. The simultaneous reduction of impurities e.g. HCP is another advantage.

 



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